Calmodulin-Dependent Protein Kinase II 290-309 is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.
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Cas No.: 115044-69-4
Sample solution is provided at 25 µL, 10mM.
Calmodulin-Dependent Protein Kinase II (290-309) is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.
Peptide 290 to 309 is found to be a potent calmodulin antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase. Neither truncation from the amino terminus (peptide 296-309) nor extension in the carboxyl-terminal direction (peptide 294-319) markedly affects calmodulin binding, whereas shortening the peptide from the carboxyl terminus (peptide 290-302) or from both ends (peptide 295-304) results in the elimination of this activity[1].
[1]. Payne ME, et al. Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. J Biol Chem. 1988 May 25;263(15):7190-5.
Kinase experiment:Ca2+/CaM-dependent phosphodiesterase is assayed at 30°C. The reaction mixture contains 40 mM Tris-HCl, pH 8.0, 5 mM magnesium acetate, 1 mM calcium chloride, 30 μM cGMP, 0.15 pCi of [3H]cGMP, 1 mM dithiothreitol, 20% glycerol, 0.64 mg/mL bovine serum albumin, 2.38 nM CaM, 50 pM CaM-deficient phosphodiesterase, and various concentrations of Calmodulin-Dependent Protein Kinase II (290-309). Following preincubation of the reaction mixture at 30°C, the reaction is initiated by the addition of substrate. After 50 min the reaction is terminated by boiling. Conditions are selected in which the reactions are linear with respect to time[1]. References: [1]. Payne ME, et al. Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. J Biol Chem. 1988 May 25;263(15):7190-5.
Cas No.115044-69-4 SDF Canonical SMILES Leu-Lys-Lys-Phe-Asn-Ala-Arg-Arg-Lys-Leu-Lys-Gly-Ala-Ile-Leu-Thr-Thr-Met-Leu-Ala Formula C 103 H 185 N 31 O 24 S M.Wt 2273.83 Solubility Soluble in DMSO Storage Store at -20°C General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
Prepare stock solution
