Reliable and robust technology for the determination of specific antibodies Immunoblot. Perfectly precise tests for confirmatory analysis.
Immunoblot kits enable the simultaneous detection of multiple markers in serum, plasma, cerebrospinal, and synovial fluid. They are an important tool for the differential diagnosis of diseases with similar symptoms. They also can be used for the confirmation of borderline or positive ELISA.
Processing can be done automatically or manually. More precise evaluation can be achieved with easy-to-use software which can be downloaded for free on our website.
An innovative antigen composition and in-house production ensure the tests are of the upmost quality with a high sensitivity and specificity.
Key benefits Innovative antigens, RTU components. Your laboratory deserves the very best!
Precise
highly specific recombinant antigens for differential diagnostics
User-friendly
ready-to-use components, quick, easy, and uniform assay procedure
Complex analysis
high number of antigens for the detection of infectious and autoimmune diseases
Easy-to-use software
fast, standardised results, elimination of subjective errors
Automatable
automated processing with open immunoblot processors
Versatile
robust and reliable test with high reproducibility of results
Highly specific recombinant antigens are bound to a nitrocellulose membrane fixed to a plastic pad. In the first reaction step, as the individual strips are incubated with the tested sample, the specific antibodies bind to the corresponding bands on the strip. After washing, the strips are incubated with a conjugate. Visualisation is performed by incubation with a substrate solution. After colour development, the test strips are dried out and evaluated using the enclosed protocol.
Control line – Validity of the test
IgA, IgG, IgM – conjugate control of respective antibody class
Start – For evaluation purposes
For test validity verification, the strips are equipped with a conjugate control band to check the presence of the appropriate conjugate and with a control band indicating kit functionality and sensitivity.
There are two major types of the kit. BLOT-LINE and BlueBLOT-LINE.
-Manual and automatic processing
Ready to use reagents:
Positive control – solution containing specific antibodies
Negative control – solution containing no specific antibodies
Conjugate – solution containing ALP labelled animal immunoglobulin
Substrate solution – buffer with BCIP and NBT
Universal solution – buffer for sample dilution and strip washing
Strips The BLOT-LINE kit contains 20 test strips. The strips are color–coded to distinguish between antibody classes:
IgG – blueIgA – greenIgM – red
Mobile template for visual evaluation Tool to assist evaluating bands without the software. The position of specific antigen bands are highlighted on the left side of the template. There is also example strip with bands displaying borderline intensity, which is on the right side of the template.
Protocol with displayed template strip For software evaluation, a protocol with self-adhesive green fields marked with a QR code is used to identify the method. Immunoblot Software is available for free on our website, contact us for more information.
- automatic processing exclusively on the device
BlueBLOT-LINE kits are intended to be performed on the BlueDiver Instrument (BDI) or its variants. These kits are the solution for automatic, simple, fast, and accurate analysis andevaluation. Kits contain only two parts, strips and ready-to-use reagent cartridges.
Strips There are 24 strips in one kit. The reactive (front) side of the strips is coated with antigens. The front side also displays a strip number and barcode for traceability of the strip after removal from the BDI. The non-reactive (back) side of the strip displays bar-coded information for identification of the strip type and lot number by the BDI.
Reagent cartridges The cartridge is composed of 7 different wells filled with ready-to-use reagents. The cartridges are sealed, and the reagent wells are hermetically separated. The back side is labelled with barcoded information for identification of cartridge type and lot number by BDI.
INFECTIOUS SEROLOGY
IMMUNOLOGY
Ing. Zuzana Gavačová
Product Specialist for Immunoblot
Testline Clinical Diagnostics
admin@frankenthalerfoundation.org
