A 13 aa peptide, n-octanoylated at Ser3, sequence from the N-terminus of mature human ghrelin (1) was coupled to KLH.
Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs act through a novel orphan G-protein coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin (”ghre” is the Proto-Indo-European root of the word “grow”) has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28aa) are produced from the 117aa precursor. In rat stomach, a 28aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake. Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects. Ghrelin administration stimulates GH secretion but also causes weight gain by increasing food intake and reduction in fat utilization and anti-Ghrelin IgG administration suppressed feeding.
Suitable for use in ELISA and Antibody Blocking Experiments. Other applications not tested.
ELISA: 50-100ng control peptide/well
Antibody Blocking Experiments: 5-10ug control peptide per 1ug of affinity pure IgG or 1ul antiserum.
Optimal dilutions to be determined by the researcher.
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human synthetic peptide|Purity: Highly purified|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2|Specificity: Sequence is 94% conserved in porcine and 84% in bovine, canine, mouse and rat ghrelins. The sequence is the same in des-ghrelins||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Sequence is 94% conserved in porcine and 84% in bovine, canine, mouse and rat ghrelins. The sequence is the same in des-ghrelins
