Xiao Feng, Shao-Qin Jian, Hao-Heng Yu, Jing-Jing Li, Ting Ma, and 5 more
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posted 20 Sep, 2021
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Background: Neuro-inflammation is regarded as one of the critical pathogenesis in neurodegenerative diseases, which is characterized by the activated microglial cells. Pectolinarin (Pec), a natural flavonoid exists in many Chinese herbal medicines, has been reported to have various biological activities. However, the effects and mechanisms on neuro-inflammation are not clear.
Methods: In this study, the inhibitory effects and mechanisms of Pec on neuro-inflammation were investigated in the LPS-stimulated microglial BV 2 cells. BV 2 microglial cells were treated with Pec or vehicle (1% DMSO), followed by LPS. ELISA, RT-PCR, NO assay, and Western blot were performed to examine the effects of Pec on neuro-inflammatory responses.
Results: We showed that Pec significantly inhibited the expression of TNF-α and IL-6 in mRNA and protein levels induced by LPS. Moreover, the production of NO, iNOS and COX-2 were suppressed by Pec in LPS-stimulated microglial BV 2 cells. In addition, Pec inhibited LPS-induced inflammation via NF-κB signaling pathway, as evidenced by reduction of the phosphorylation of IKK, the degradation of IκBα and the nuclear translocation of p65.
Conclusions: Taken together, Pec exhibited anti-inflammatory effects in LPS-stimulated microglial BV 2 cells via NF-κB signaling pathway, which might provide therapeutic potential for neuro-inflammation and neurodegenerative diseases.
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