Helen Frankenthaler Foundation

Phosphorylase Kinase beta subunit peptide

Plain Text Title: Tachykinin-related peptide, TRP2

Product Information

Product Name: Tachykinin-related peptide, TRP2

Documents:

Sequence Shortening: ALMGFQGVR-amide

Sequence: H-Ala-Leu-Met-Gly-Phe-Gln-Gly-Val-Arg-NH2

Length (aa): 9

Peptide Purity (HPLC): 95.8%

Molecular Formula: C 43 H 71 N 13 O 11 S

Molecular Weight: 977.18

Source: Synthetic

Form: Powder

Description: Tachykinin-related peptide 2 (TRP2) is a neuropeptide identified in the honeybee Apis mellifera. It is one of seven tachykinin-related peptides (AmTRP1-7) encoded by the prepro-AmTRP gene. The peptide has the amino acid sequence ALMGFQGVRamide and possesses the C-terminal motif FX 1 GX 2 Ramide, which is characteristic of invertebrate tachykinin-related peptides. Expression analysis of the prepro-AmTRP gene indicates differential regulation in honeybee castes. Transcript levels are highest in the heads of forager bees and queens, lower in nurse bees, and nearly absent in drones. This expression pattern suggests the peptide may function as a neuromodulator or hormone associated with age-related division of labor and sex-specific physiology in honeybees.

Storage Guidelines: Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides.

References

  • Takeuchi H, Yasuda A, Yasuda-kamatani Y, Kubo T, Nakajima T. Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads. Insect Mol Biol. 2003;12(3):291-8.
  • Han B, Wei Q, Wu F, Hu H, Ma C, Meng L, Zhang X, Feng M, Fang Y, Rueppell O, Li J. Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers. Elife. 2021 Mar 24;10:e64830. doi: 10.7554/eLife.64830. PMID: 33760729; PMCID: PMC8016481.

About TFA salt

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2 H, 15 N, and 13 C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.