Receptor

Melanocortin 2 (MC2R, ACTHR) Receptor Reporter Assay System

A cell-based luciferase reporter assay kit targeting the Human Melanocortin 2 Receptor

Features

• Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against MC2R
• Robust technology provides sustained response for consistent and reproducible data acquisition
• Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate

Technical Information

Shipping & Storage Information

Storage

• -80°C

Shipping

• Dry ice in continental US; may vary elsewhere

Product Description

INDIGO’s Melanocortin 2 (MC2R, ACTHR) receptor assay utilizes proprietary human cells that have been engineered to provide constitutive expression of the Human Melanocortin type 2 Receptor (MC2R, ACTHR). In addition to MC2R Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate.

The melanocortin type 2 receptor (MC2R) is one of five members of the Melanocortin receptors (MCR). Unlike other types of MCR, MC2R, also known as the Adrenocorticotropin Hormone Receptor (ACTHR), exclusively binds ACTH and mediates its function in steroidogenesis and the release of cortisol. The melanocortin association protein (MRAP) is required for the transport of MC2R to the cell membrane and for binding ACTH and mediating its response. Familial glucocorticoid deficiency, Cushing’s syndrome and Addison disease are examples of diseases linked to mutations in MC2R or the accessory protein MRAP leading to imbalance in the blood levels of ACTH and cortisol . MC2R is predominantly expressed in the adrenal cortex, but can also