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(SC-20) In-line Heater/Cooler

(SC-20) In-line Heater/Cooler

SKU: GRP-0000217

This dual in-line solution heater/cooler extends the temperature range for controlled perfusion studies of both coverslip mounted cells and anchored tissue thin slices.

  • Heats or cools perfusate*
  • Compatible with all Warner Series 20 chambers
  • Requires a CL-100 or CL-200A Bipolar Temperature Controller and LCS-1 Heat Exchanger
  • Versatile and simple to use thermal control device

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Grouped Product Items

Item No.Model No.DescriptionQty
64-0353SC-20Solution heater / cooler, dual channelPlease login to buy
64-0352CL-100Bipolar temperature controllerPlease login to buy
64-1922LCS-1Liquid Cooling DevicePlease login to buy
64-0354PHC-1Heater/Cooler jacket for Series 20 chambers (not compatible with inverted microscopes)Please login to buy
64-0355PHC-2Heater/Cooler jacket for diamond bath Series 20 chambersPlease login to buy

Details

In-line solution heating has proven to be one of the most effective methods of maintaining the temperature of perfusion solutions. The SC-20 Dual In-line Solution Heater/Cooler utilizes Peltier thermoelectric technology to regulate temperature both above and below ambient levels.

The SC-20 is designed to thermally regulate either one or two solutions at the same temperature. Temperature regulation is provided by the CL-200A or CL-100 Bipolar Temperature controller.

Solution temperatures can be maintained at 0°C at flow rates of 2 ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min.

An integral water jacket is used to remove excess heat from the SC-20 and the LCS-1 is specifically designed to provide this service.

The SC-20 can be used with either one or two discrete perfusate solutions, or with a solution/gas combination. When coupled with a PHC Series Imaging Chamber Heater/Cooler Jacket, the SC-20 provides an effective means of temperature control in the chamber, even under slow-flow conditions.

* Be advised that saturation limits for many ionic compounds are decreased at lower temperatures and that this is not a property of the SC-20 or other Warner apparatus.

Each heater/cooler is supplied with a TA-29 thermistor cable assembly for monitoring the bath temperature.

References

  • The Effect of Thermal Acclimation on Action Potentials and Sarcolemmal K+ Channels from Pacific Bluefin Tuna Cardiomyocytes. Galli GL, Lipnick MS, Block BA. Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R502-9
  • Temporal Dynamics of Neuronal Activation by Channelrhodopsin-2 and TRPA1 Determine Behavioral Output in Drosophila Larvae. Pulver SR, Pashkovski SL, Hornstein NJ, Garrity PA, Griffith LC. J Neurophysiol. 2009 Jun;101(6):3075-88
  • Pore region of TRPV3 ion channel is specifically required for heat activation. Grandl J, Hu H, Bandell M, Bursulaya B, Schmidt M, Petrus M, Patapoutian A. Nat Neurosci. 2008 Sep;11(9):1007-13
  • Identification of molecular determinants of channel gating in the transient receptor potential box of vanilloid receptor I. Valente P, García-Sanz N, Gomis A, Fernández-Carvajal A, Fernández-Ballester G, Viana F, Belmonte C, Ferrer-Montiel A. FASEB J. 2008 Sep;22(9):3298-309
  • An internal thermal sensor controlling temperature preference in Drosophila. Hamada FN, Rosenzweig M, Kang K, Pulver SR, Ghezzi A, Jegla TJ, Garrity PA. Nature. 2008 Jul 10;454(7201):217-20
  • The TRPV3 mutation associated with the hairless phenotype in rodents is constitutively active. Xiao R, Tian J, Tang J, Zhu MX. Cell Calcium. 2008 Apr;43(4):334-43
  • Highly Ca2+-selective TRPM channels regulate IP3-dependent oscillatory Ca2+ signaling in the C. elegans intestine. Xing J, Yan X, Estevez A, Strange K. J Gen Physiol. 2008 Mar;131(3):245-55
  • Divergent Biophysical Defects Caused by Mutant Sodium Channels in Dilated Cardiomyopathy With Arrhythmia. Nguyen TP, Wang DW, Rhodes TH, George AL Jr. Circ Res. 2008 Feb 15;102(3):364-71
  • A Role of the Transient Receptor Potential Domain of Vanilloid Receptor I in Channel Gating. García-Sanz N, Valente P, Gomis A, Fernández-Carvajal A, Fernández-Ballester G, Viana F, Belmonte C, Ferrer-Montiel A. J Neurosci. 2007 Oct 24;27(43):11641-50
  • In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space. Thorne RG, Nicholson C. Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5567-72
  • Diurnal and estradiol-dependent changes in gonadotropin-releasing hormone neuron firing activity. Christian CA, Mobley JL, Moenter SM. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15682-7
  • Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion. White FA, Sun J, Waters SM, Ma C, Ren D, Ripsch M, Steflik J, Cortright DN, Lamotte RH, Miller RJ. Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14092-7
  • Subtype-Specific GABA Transporter Antagonists Synergistically Modulate Phasic and Tonic GABAA Conductances in Rat Neocortex. Keros S, Hablitz JJ. J Neurophysiol. 2005 Sep;94(3):2073-85
  • High-Concentration Rapid Transients of Glutamate Mediate Neural-Glial Communication via Ectopic Release. Matsui K, Jahr CE, Rubio ME. J Neurosci. 2005 Aug 17;25(33):7538-47
  • Biphasic Currents Evoked by Chemical or Thermal Activation of the Heat-gated Ion Channel, TRPV3*. Chung MK, Güler AD, Caterina MJ. J Biol Chem. 2005 Apr 22;280(16):15928-41

Specifications

ParameterSpecification
Minimum Temperature0°C at 2 mL/min maximum flow
Maximum Temperature50°C
Maximum Flow Rate at 5°C5 mL/min
Accuracy± 0.1°C
Internal Dead Volume330 µL
ControllerModel CL-100 Bipolar Controller
Perfusion LinesType 316 Stainless Steel 0.032 ID x 0.062 OD
Water Jacket PortsType 316 Stainless Steel 0.12 ID