ab209792
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6 Publications
Rabbit Recombinant Monoclonal GIP antibody. Suitable for mIHC, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Gastric inhibitory polypeptide, GIP, Glucose-dependent insulinotropic polypeptide, Incretin hormone
Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Sporadic and cytoplasmic staining on human duodenum is observed (PMID : 4574902).
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Carboxypeptidase A (gray; Opal™690), anti-GIP (green; Opal™520) and anti-Somatostatin 28 (red; Opal™570) on human pancreas. Panel B : anti-Carboxypeptidase A stained on acinar cells. Panel C : anti-GIP stained on alpha cells. Panel D : anti-Somatostatin 28 stained on delta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab278044 at 1/4000 dilution (0.135 μg/ml), ab271989 at 1/4000 dilution (0.27 μg/ml) and ab111912 at 1/9000 dilution (0.068 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This data was developed using ab271989, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on alpha cells of human pancreas islet is observed (PMID : 4574902).
Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling GIP with ab209792 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on alpha cells of mouse pancreas islet is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Sporadic and cytoplasmic staining on mouse small intestine is observed (PMID : 4574902).
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on alpha cells of rat pancreas islet is observed (PMID : 4574902).
Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).
Panel A : merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
Panel B : anti-Somatostatin 28 staining the delta cells in rat pancreas islet.
Panel C : anti-GIP staining the alpha cells in rat pancreas islet.
Panel D : anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.
Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using ab271989, the same antibody clone in a different buffer formulation.
Panel A : merged staining of anti-SV2A (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
Panel B : anti-SV2A staining predominantly the beta cells in mouse pancreas islet.
Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab254351 at 1/1000 dilution (0.48 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining MafA with ab324869 at a 1 : 100 (5.13 ug/ml) dilution, ab181547 anti-Insulin used at 1 : 20000 (0.053 ug/ml) d
